Journal: Journal of Biochemical and Molecular Toxicology
Article Title: Elevated BCRP Transporter and Altered NF‐кB Pathway Mediate Zoledronic Acid Resistance in MCF‐7 Cells
doi: 10.1002/jbt.70397
Figure Lengend Snippet: Pheophorbide a (PhA) accumulation in MCF‐7 parental and MCF‐7/Zol cells (a) Histogram demonstrates the accumulation curves in the absence and presence of the potent inhibitor elacridar (b) Median fluorescence values were used to determine the fold changes in PhA accumulation in comparison to that of untreated MCF‐7/S cells. (***) Indicates significance ( p ≤ 0.0001) between the groups. Error bars represent SE.
Article Snippet: To determine the BCRP pump activity in cells, the intracellular concentration of the fluorescent dye Pheophorbide a (PhA) (#16072, Cayman Chemical, USA), a substrate‐specific to BCRP, was measured by flow cytometry as described by Robey et al. [ ].
Techniques: Fluorescence, Comparison